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kinA [2017-07-13 10:11:45]
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kinA [2017-07-13 10:11:45]

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
locus
BSU13990
pI
5.49
mw
68.99 kDa
protein length
606 aa Sequence Blast
gene length
1818 bp Sequence Blast
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
spoIIF,spoIIJ,gsiC,scoD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    1,470,026 → 1,471,846

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]
  • mainly active in the older, inner regions of a colony (with [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB]) [Pubmed|21097618]

    • [SW|Domains]

  • three tandem [SW|PAS domain]s in the N-terminal region of KinA, the [SW|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
  • the first [SW|PAS domain] is required for NAD(+) binding [Pubmed|23599347]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • interaction with [protein|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|PxpB] or [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|Sda] inhibits autophosphorylation of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] [Pubmed|9334321,15023339]
  • autophosphorylation is inhibited by [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|SivA] and [protein|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|BslA] [Pubmed|23335417]
  • activated by interaction with NAD(+) that indicated poor respiratory activity of the cell [Pubmed|23599347], this has been falsified [pubmed|28449380]

    • Structure

  • [PDB|2VLG] (PAS domain)

    • [SW|Localization]

  • cytoplasm

    • additional information

  • the protein forms tetramers [pubmed|28449380]

    • Expression and Regulation

    Operons

    genes
    [gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]
    description
    [Pubmed|1569009]

    sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|1569009], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|9644251], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [regulon|stringent response|stringent response]: positive regulation, in [regulon|stringent response|stringent response]

    • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|9644251]

    • additional information

  • promoter-up mutations or high artificial expression that render ''[SW|kinA]'' independent from [SW|SigH], allow [SW|sporulation] at high salinity [Pubmed|26712348]
    • view in new tab

    References

    Reviews

  • 22303284

    • Original Publications

  • 23169620,20511506,19561131,20413551,1846779,11292798,16166384,11734624,9299348,12067336,18174125,10094672,11069677,15023339,2509430,17350039,9334321,9477965,9644251,1569009,20081035,21097618,21926229,22303282,22670053,23335417,23378509,23504013,23599347,25598361,26055117,26165942,26657919,26712348,27216630,28449380,18324779